AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test Method

AOAC Germicidal and Detergent Sanitizing Action of Disinfectants (AOAC 960.09)

Welcome to Antimicrobial Test Laboratories' AOAC Germicidal and Detergent Sanitizing Action of Disifnectants Test Method Resource Page.

Below, you will find a summary of the Germicidal and Detergent Sanitizing Action of Disifnectants Test Method along with some of its strengths and weaknesses. The Germicidal and Detergent Sanitizing Action of Disifnectants Test Method is specified by US EPA as a method which can be used to substantiate efficacy claims for certain food-contact surface sanitizers. It is particularly appropriate for dilutable sanitizers that do not utilize halogens (chlorine, bromine).

Note: The information below is presented for educational purposes only. Current, detailed AOAC Methods can be obtained from AOAC.

Test Method Summary:

Cultures of a AOAC-specified Gram-negative and Gram-positive bacterium are grown in appropriate broth, and then concentrated to approximately 1 x 10^10 cfu/ml.
In duplicate, for each microorganism, 1 ml of the concentrated culture is inoculated into an Erlenmeyer flask containing 99 ml of the test product, for the specified contact time.
Separately, 1 ml of the concentrated culture is inoculated into an Erlenmeyer flask containing 99 ml of a non-germicidal buffer, to determine the initial concentration of microorganisms.
After the contact time has elapsed (usually 30 seconds), aliquots of the microbe/product mixture are removed from the flask and analyzed for surviving microorganisms by dilution and plating, coupled with an appropriate chemical neutralization strategy.
Agar plates are incubated for a sufficient period.
After incubation, the concentration of surviving microorganisms in the test flasks is compared to that in the control (initial concentration) flasks.
If the product kills >99.999% of both the Gram-positive and Gram-negative test microorganism, then it passes the test.

Strengths of the AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test:

The AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test is highly reproducible.
The AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test is a great evaluation of disinfection performance in the presence of excess liquid, such as might be expected for "bulk-type" food contact sanitizing rinses (think drinking glass rinse at a bar).

Weaknesses of the AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test:

The AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test is somewhat removed from "real-life" hard surface sanitization (trigger sprayers, aerosols, etc).

Antimicrobial Test Laboratories has put a great deal of time and thought into perfecting the way we execute the The AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test. We enjoy performing this particular sanitizer test because the high degree of reproducibility makes comparisons between technologies more reliable.

For more information about the AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test, Contact the Lab Today!